Iain Fraser Cytometry Centre (Core Facility)

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RRID:SCR_022315

 

The Iain Fraser Cytometry Centre (IFCC) is a multi-user GMO and BSL2-approved cytometry facility. The mission of IFCC is to provide investigators access to well-maintained instrumentation and human domain expertise for successful research outcomes. The IFCC gratefully acknowledges the following funding sources for equipment acquisitions: Dr Iain Fraser, University of Aberdeen, Saving Sight for Grampian, SULSA, Friends of Anchor, and The Gordon and Emily Baxter Foundation.

Dr Andrea Holme manages the facility and will be pleased to discuss collaborations and projects. A bespoke Grant Support Letter can be provided. Don't hesitate to get in touch with Dr Holme.

 

The IFCC staff are active members of the Scottish Cytometry Society (SSC), the International Society for the Advancement of Cytometry (ISAC), FlowUK (Royal Microscopy Society), and Core Technologies for Life Sciences (CTLS) We welcome staff shadowing and placements.

 

Access Terms and Conditions: 

  • Users must have an active IFCC iLab Profile;
  • All experiments must comply with the biosafety regulations, IFCC and University Policy;
  • Only Core Staff can train users on the instruments and book instruments for untrained users.
  • Samples must be transported in a double-sealed container and capped tubes used. This protects both the samples and the public.

  

 -submit a contact request form 

OR email Cytometry Service Contact <p01ry4@abdn.ac.uk>

 

Acknowledgement Policy

Please reference the Facility's Research Resource Identifier RRID:SCR_022315

1.     A publication including data (main figures or supplemental) generated within the IFCC, stating: “We acknowledge the Iain Fraser Cytometry Center for assistance with the generation of Cytometry data”. 

3.     If an IFCC member worked closely with you on a project, you should acknowledge this. It is good practice to include their name in the acknowledgement section, at least.

4.    When intellectual input is given beyond basic training, you can offer staff a co-authorship. This is a critical consideration as a project evolves. Our resource staff is asked to contribute knowledge that makes a publication possible or enhances its value (novel resource laboratory work and insight, experiment design, preparation, data production & analysis). This should be discussed at the earliest opportunity.

5.     You can use our logo in a talk or a poster for a conference. This is intended to be for acknowledgement purposes.

 

Suggested Wording: The authors acknowledge the Iain Fraser Cytometry Centre (RRID:SCR_022315) for assistance with [Insert what we supported here].

 

 

Frequently Asked Questions

0. I have forgotten my password or have difficulties with  iLab: iLab provides 24/7 support and if you have any issues with your account, contact them by e-mail:ilab-support@agilent.com

1.  What Training do you have, and how do I start a request for it?   

    1. Register for an iLab account- you only need to fill in the * sections.
    2. Submit a Profile Registration request through iLab (Training and Instrument Access). This will trigger training and service meetings to schedule your bespoke support. 
    3. Hands-on instrument training competency evaluation Fortessa or Attune (pdf)

Under normal circumstances, hands-on instrument training time is not shared between people-only one person can be trained per session.  New users are given access to online training resources, which will enable them to commence their training even while working remotely. 

2. How do I book machine time, training, or sorting?

    1. Register for an iLab account: Click on the Registration button located on the top right of this page (or click here) and follow the instructions (here). 
    2. Requesting training and services will only be possible once you have an iLab account and log into the IFCC iLab site.
    3. To Book on the iLab instrument Calendar: Click, hold, drag and let go of the left-click button of the mouse, then follow the on-screen instructions.
    4. University Out-of-Hours Usage: Don't hesitate to contact IFCC staff regarding out-of-hours usage. An  "Out of hours" form must be completed.
    5. Data Analysis: The computers can be booked and used remotely. The dongles do not leave the facility.
    6. Staff Instrument-based Assistance: Select "technical assistance" when booking. At least 72 hours' notice is preferred.  For urgent inquiries, contact cytometry@abdn.ac.uk.

Core Staff Hours: Monday-Friday, 9:00 am - 5:00 pm. Experienced users may also request out-of-hours use of the instruments.

3. What Instruments do you have?  

BD LSR Fortessa, Attune NxT with 96/384 well plate HTS system, Dual Camera Amnis ImageStream MKII with 96 well plate loader, BD Influx, BioPlex 200, GentleMACs dissociator, and bespoke data analysis software. The laboratory also houses a biosafety cabinet, fume cupboard, Thermo Scientific™ Megafuge 16R- FACS Tubes, 96 well plates, 15 and 50 ml tubes, an Eppendorf tube centrifuge, a magnetic plate holder, a shaker, an inverted phase-contrast microscope and tissue culture suites for mammalian and microbial culturing.  

3. What Services do you offer?

The core has a dedicated tissue culture suit and wet bench laboratory facilities to support wet bench work pipelines for your experimental needs. 

The Facility operates on a fee-for-use basis following university recharge policies. Access to the IFCC facility is provided to University of Aberdeen faculty and staff, external academics, for-profit companies and commercial scientists through fee-for-service, project basis, partnerships, and collaboration.

Note: Fluorescent Activated Cell Sorting (FACS): The cell sorter is core staff-operated only. All experiments must be discussed and scheduled with IFCC staff in advance. For Registered users, submit a short request here.

4. Can the Core stain and run my samples:  If you do not want to be trained and run the instrument yourself, the expert staff can undertake the work for you with an additional hourly charge. Contact andrea.holme@abdn.ac.uk

5. Pricing Eligibility criteria:

For more information, contact andrea.holme@abdn.ac.uk.

  • Internal Rates: Apply to UofA Staff, Students, and Affiliates (including Adjunct, Clinical, and Honorary appointments), when the project grant funding is administered by UofA.
  • External/Transparent Approach to Costing (TRAC) Rates: Apply when the IFCC facility access is for a project with funding administered by another organization or has been commissioned by the funder.
  • Commercial Rates: Apply to commercial and industry-based projects. 

 

Contacts

Name Role Phone Email Location
Iain Fraser Cytometry Centre
Centre
 
+44(0)1224-437597
 
cytometry@abdn.ac.uk
 
University of Aberdeen
 

Services and Consumables


Search available services: View: by category alphabetically
FACS Cell Sorting (BD Influx) (1)
Sample Processing By Core Staff (1)

Available Equipment and Resources

Cell Sorter- IMS- Lab 1.38.1 (1)
Cytometry Instruments-IMS-2.54 (5)
Fume Cupboards, Auxiliary Equipment, Visits, Sample prep bench time (4)
Off line (1)
Remote Access for Data Analysis Tools (1)

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